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paper n a plko 1 shnt addgene  (Addgene inc)


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    Addgene inc paper n a plko 1 shnt addgene
    Paper N A Plko 1 Shnt Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paper+n+a+plko+1+shnt+addgene/pLKO%2E1-puro-shNT+(Plasmid+%23109012)/pm41076632-47-166-169
    Average 93 stars, based on 11 article reviews
    paper n a plko 1 shnt addgene - by Bioz Stars, 2026-10
    93/100 stars

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    Virus:

    Article Title: Protocol for monitoring the stability of transcription factor EB using global protein stability assay.
    Article Snippet: Bacterial and virus strains E. coli DH5α cells Thermo Fisher Scientific Cat# 18265017 Chemicals, peptides, and recombinant proteins Penicillin/Streptomycin Thermo Fisher Scientific Cat# 15070063 Fetal bovine serum Sigma-Aldrich Cat# F0193 DMEM Metacell Cat# C1010 RPMI1640 Metacell Cat# C1120 0.25% Trypsin-EDTA solution Metacell Cat# C1201 Liposomal Transfection Reagent Yeasen Cat# 40802ES03 Polybrene Beyotime Cat# C0351 Puromycin dihydrochloride Beyotime Cat# ST551 Blasticidin S HCl Beyotime Cat# ST018 DAPI dihydrochloride MedChemExpress Cat# HY-D0814 Critical commercial assays Seamless Cloning Kit Beyotime Cat# D7010 MolPure Gel Extraction Kit Yeasen Cat# 19101ES50 Hieff Canace High-Fidelity DNA Polymerase Yeasen Cat# 10135ES60 MolPure Endo-free Plasmid Mini Kit Yeasen Cat# 19021ES50 23 SuperTaq PCR StarMix (dye) GenStar Cat# A002-10 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 NCI-H1299 ATCC Cat# CRL-5803 Oligonucleotides Seamless Cloning TFEB forward primer: 5′ TCCATAGAAGCCACCATGGCGTCACGCATAGGG 3′ This paper N/A Seamless Cloning TFEB reverse primer: 5′ ATCGAATTCTCTAGACTTGTCGTCATCGTCTTTG 3′ This paper N/A Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCDH-CMV-MCS-GFP-IRES-RFP Modified from Addgene pCDH-Flag-c-Myc Addgene Plasmid Cat# 102626 pCDH-CMV-MCS-GFP-TFEB-IRES-RFP This paper N/A PLKO.1-shNT Addgene Cat# 109012 PLKO.1-shYEATS2 Wang et al. N/A Software and algorithms FlowJo v.10 BD Biosciences https://www.flowjo.com Other 0.45 μm filters BIOFIL Cat# FPE404025 XbaI Beyotime Cat# D6713 EcoRI Beyotime Cat# D6330 BD FACSCanto II BD Biosciences N/A STAR Protocols 6, 104141, December 19, 2025 3 Generate cell lines stably expressing pCDH-CMV-TFEB-GFP-IRES-RFP through lentiviral transduction.

    Recombinant:

    Article Title: Protocol for monitoring the stability of transcription factor EB using global protein stability assay.
    Article Snippet: Bacterial and virus strains E. coli DH5α cells Thermo Fisher Scientific Cat# 18265017 Chemicals, peptides, and recombinant proteins Penicillin/Streptomycin Thermo Fisher Scientific Cat# 15070063 Fetal bovine serum Sigma-Aldrich Cat# F0193 DMEM Metacell Cat# C1010 RPMI1640 Metacell Cat# C1120 0.25% Trypsin-EDTA solution Metacell Cat# C1201 Liposomal Transfection Reagent Yeasen Cat# 40802ES03 Polybrene Beyotime Cat# C0351 Puromycin dihydrochloride Beyotime Cat# ST551 Blasticidin S HCl Beyotime Cat# ST018 DAPI dihydrochloride MedChemExpress Cat# HY-D0814 Critical commercial assays Seamless Cloning Kit Beyotime Cat# D7010 MolPure Gel Extraction Kit Yeasen Cat# 19101ES50 Hieff Canace High-Fidelity DNA Polymerase Yeasen Cat# 10135ES60 MolPure Endo-free Plasmid Mini Kit Yeasen Cat# 19021ES50 23 SuperTaq PCR StarMix (dye) GenStar Cat# A002-10 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 NCI-H1299 ATCC Cat# CRL-5803 Oligonucleotides Seamless Cloning TFEB forward primer: 5′ TCCATAGAAGCCACCATGGCGTCACGCATAGGG 3′ This paper N/A Seamless Cloning TFEB reverse primer: 5′ ATCGAATTCTCTAGACTTGTCGTCATCGTCTTTG 3′ This paper N/A Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCDH-CMV-MCS-GFP-IRES-RFP Modified from Addgene pCDH-Flag-c-Myc Addgene Plasmid Cat# 102626 pCDH-CMV-MCS-GFP-TFEB-IRES-RFP This paper N/A PLKO.1-shNT Addgene Cat# 109012 PLKO.1-shYEATS2 Wang et al. N/A Software and algorithms FlowJo v.10 BD Biosciences https://www.flowjo.com Other 0.45 μm filters BIOFIL Cat# FPE404025 XbaI Beyotime Cat# D6713 EcoRI Beyotime Cat# D6330 BD FACSCanto II BD Biosciences N/A STAR Protocols 6, 104141, December 19, 2025 3 Generate cell lines stably expressing pCDH-CMV-TFEB-GFP-IRES-RFP through lentiviral transduction.

    Transfection:

    Article Title: Protocol for monitoring the stability of transcription factor EB using global protein stability assay.
    Article Snippet: Bacterial and virus strains E. coli DH5α cells Thermo Fisher Scientific Cat# 18265017 Chemicals, peptides, and recombinant proteins Penicillin/Streptomycin Thermo Fisher Scientific Cat# 15070063 Fetal bovine serum Sigma-Aldrich Cat# F0193 DMEM Metacell Cat# C1010 RPMI1640 Metacell Cat# C1120 0.25% Trypsin-EDTA solution Metacell Cat# C1201 Liposomal Transfection Reagent Yeasen Cat# 40802ES03 Polybrene Beyotime Cat# C0351 Puromycin dihydrochloride Beyotime Cat# ST551 Blasticidin S HCl Beyotime Cat# ST018 DAPI dihydrochloride MedChemExpress Cat# HY-D0814 Critical commercial assays Seamless Cloning Kit Beyotime Cat# D7010 MolPure Gel Extraction Kit Yeasen Cat# 19101ES50 Hieff Canace High-Fidelity DNA Polymerase Yeasen Cat# 10135ES60 MolPure Endo-free Plasmid Mini Kit Yeasen Cat# 19021ES50 23 SuperTaq PCR StarMix (dye) GenStar Cat# A002-10 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 NCI-H1299 ATCC Cat# CRL-5803 Oligonucleotides Seamless Cloning TFEB forward primer: 5′ TCCATAGAAGCCACCATGGCGTCACGCATAGGG 3′ This paper N/A Seamless Cloning TFEB reverse primer: 5′ ATCGAATTCTCTAGACTTGTCGTCATCGTCTTTG 3′ This paper N/A Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCDH-CMV-MCS-GFP-IRES-RFP Modified from Addgene pCDH-Flag-c-Myc Addgene Plasmid Cat# 102626 pCDH-CMV-MCS-GFP-TFEB-IRES-RFP This paper N/A PLKO.1-shNT Addgene Cat# 109012 PLKO.1-shYEATS2 Wang et al. N/A Software and algorithms FlowJo v.10 BD Biosciences https://www.flowjo.com Other 0.45 μm filters BIOFIL Cat# FPE404025 XbaI Beyotime Cat# D6713 EcoRI Beyotime Cat# D6330 BD FACSCanto II BD Biosciences N/A STAR Protocols 6, 104141, December 19, 2025 3 Generate cell lines stably expressing pCDH-CMV-TFEB-GFP-IRES-RFP through lentiviral transduction.

    Cloning:

    Article Title: Protocol for monitoring the stability of transcription factor EB using global protein stability assay.
    Article Snippet: Bacterial and virus strains E. coli DH5α cells Thermo Fisher Scientific Cat# 18265017 Chemicals, peptides, and recombinant proteins Penicillin/Streptomycin Thermo Fisher Scientific Cat# 15070063 Fetal bovine serum Sigma-Aldrich Cat# F0193 DMEM Metacell Cat# C1010 RPMI1640 Metacell Cat# C1120 0.25% Trypsin-EDTA solution Metacell Cat# C1201 Liposomal Transfection Reagent Yeasen Cat# 40802ES03 Polybrene Beyotime Cat# C0351 Puromycin dihydrochloride Beyotime Cat# ST551 Blasticidin S HCl Beyotime Cat# ST018 DAPI dihydrochloride MedChemExpress Cat# HY-D0814 Critical commercial assays Seamless Cloning Kit Beyotime Cat# D7010 MolPure Gel Extraction Kit Yeasen Cat# 19101ES50 Hieff Canace High-Fidelity DNA Polymerase Yeasen Cat# 10135ES60 MolPure Endo-free Plasmid Mini Kit Yeasen Cat# 19021ES50 23 SuperTaq PCR StarMix (dye) GenStar Cat# A002-10 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 NCI-H1299 ATCC Cat# CRL-5803 Oligonucleotides Seamless Cloning TFEB forward primer: 5′ TCCATAGAAGCCACCATGGCGTCACGCATAGGG 3′ This paper N/A Seamless Cloning TFEB reverse primer: 5′ ATCGAATTCTCTAGACTTGTCGTCATCGTCTTTG 3′ This paper N/A Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCDH-CMV-MCS-GFP-IRES-RFP Modified from Addgene pCDH-Flag-c-Myc Addgene Plasmid Cat# 102626 pCDH-CMV-MCS-GFP-TFEB-IRES-RFP This paper N/A PLKO.1-shNT Addgene Cat# 109012 PLKO.1-shYEATS2 Wang et al. N/A Software and algorithms FlowJo v.10 BD Biosciences https://www.flowjo.com Other 0.45 μm filters BIOFIL Cat# FPE404025 XbaI Beyotime Cat# D6713 EcoRI Beyotime Cat# D6330 BD FACSCanto II BD Biosciences N/A STAR Protocols 6, 104141, December 19, 2025 3 Generate cell lines stably expressing pCDH-CMV-TFEB-GFP-IRES-RFP through lentiviral transduction.

    Gel Extraction:

    Article Title: Protocol for monitoring the stability of transcription factor EB using global protein stability assay.
    Article Snippet: Bacterial and virus strains E. coli DH5α cells Thermo Fisher Scientific Cat# 18265017 Chemicals, peptides, and recombinant proteins Penicillin/Streptomycin Thermo Fisher Scientific Cat# 15070063 Fetal bovine serum Sigma-Aldrich Cat# F0193 DMEM Metacell Cat# C1010 RPMI1640 Metacell Cat# C1120 0.25% Trypsin-EDTA solution Metacell Cat# C1201 Liposomal Transfection Reagent Yeasen Cat# 40802ES03 Polybrene Beyotime Cat# C0351 Puromycin dihydrochloride Beyotime Cat# ST551 Blasticidin S HCl Beyotime Cat# ST018 DAPI dihydrochloride MedChemExpress Cat# HY-D0814 Critical commercial assays Seamless Cloning Kit Beyotime Cat# D7010 MolPure Gel Extraction Kit Yeasen Cat# 19101ES50 Hieff Canace High-Fidelity DNA Polymerase Yeasen Cat# 10135ES60 MolPure Endo-free Plasmid Mini Kit Yeasen Cat# 19021ES50 23 SuperTaq PCR StarMix (dye) GenStar Cat# A002-10 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 NCI-H1299 ATCC Cat# CRL-5803 Oligonucleotides Seamless Cloning TFEB forward primer: 5′ TCCATAGAAGCCACCATGGCGTCACGCATAGGG 3′ This paper N/A Seamless Cloning TFEB reverse primer: 5′ ATCGAATTCTCTAGACTTGTCGTCATCGTCTTTG 3′ This paper N/A Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCDH-CMV-MCS-GFP-IRES-RFP Modified from Addgene pCDH-Flag-c-Myc Addgene Plasmid Cat# 102626 pCDH-CMV-MCS-GFP-TFEB-IRES-RFP This paper N/A PLKO.1-shNT Addgene Cat# 109012 PLKO.1-shYEATS2 Wang et al. N/A Software and algorithms FlowJo v.10 BD Biosciences https://www.flowjo.com Other 0.45 μm filters BIOFIL Cat# FPE404025 XbaI Beyotime Cat# D6713 EcoRI Beyotime Cat# D6330 BD FACSCanto II BD Biosciences N/A STAR Protocols 6, 104141, December 19, 2025 3 Generate cell lines stably expressing pCDH-CMV-TFEB-GFP-IRES-RFP through lentiviral transduction.

    Plasmid Preparation:

    Article Title: Protocol for monitoring the stability of transcription factor EB using global protein stability assay.
    Article Snippet: Bacterial and virus strains E. coli DH5α cells Thermo Fisher Scientific Cat# 18265017 Chemicals, peptides, and recombinant proteins Penicillin/Streptomycin Thermo Fisher Scientific Cat# 15070063 Fetal bovine serum Sigma-Aldrich Cat# F0193 DMEM Metacell Cat# C1010 RPMI1640 Metacell Cat# C1120 0.25% Trypsin-EDTA solution Metacell Cat# C1201 Liposomal Transfection Reagent Yeasen Cat# 40802ES03 Polybrene Beyotime Cat# C0351 Puromycin dihydrochloride Beyotime Cat# ST551 Blasticidin S HCl Beyotime Cat# ST018 DAPI dihydrochloride MedChemExpress Cat# HY-D0814 Critical commercial assays Seamless Cloning Kit Beyotime Cat# D7010 MolPure Gel Extraction Kit Yeasen Cat# 19101ES50 Hieff Canace High-Fidelity DNA Polymerase Yeasen Cat# 10135ES60 MolPure Endo-free Plasmid Mini Kit Yeasen Cat# 19021ES50 23 SuperTaq PCR StarMix (dye) GenStar Cat# A002-10 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 NCI-H1299 ATCC Cat# CRL-5803 Oligonucleotides Seamless Cloning TFEB forward primer: 5′ TCCATAGAAGCCACCATGGCGTCACGCATAGGG 3′ This paper N/A Seamless Cloning TFEB reverse primer: 5′ ATCGAATTCTCTAGACTTGTCGTCATCGTCTTTG 3′ This paper N/A Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCDH-CMV-MCS-GFP-IRES-RFP Modified from Addgene pCDH-Flag-c-Myc Addgene Plasmid Cat# 102626 pCDH-CMV-MCS-GFP-TFEB-IRES-RFP This paper N/A PLKO.1-shNT Addgene Cat# 109012 PLKO.1-shYEATS2 Wang et al. N/A Software and algorithms FlowJo v.10 BD Biosciences https://www.flowjo.com Other 0.45 μm filters BIOFIL Cat# FPE404025 XbaI Beyotime Cat# D6713 EcoRI Beyotime Cat# D6330 BD FACSCanto II BD Biosciences N/A STAR Protocols 6, 104141, December 19, 2025 3 Generate cell lines stably expressing pCDH-CMV-TFEB-GFP-IRES-RFP through lentiviral transduction.

    Polymerase Chain Reaction:

    Article Title: Protocol for monitoring the stability of transcription factor EB using global protein stability assay.
    Article Snippet: Bacterial and virus strains E. coli DH5α cells Thermo Fisher Scientific Cat# 18265017 Chemicals, peptides, and recombinant proteins Penicillin/Streptomycin Thermo Fisher Scientific Cat# 15070063 Fetal bovine serum Sigma-Aldrich Cat# F0193 DMEM Metacell Cat# C1010 RPMI1640 Metacell Cat# C1120 0.25% Trypsin-EDTA solution Metacell Cat# C1201 Liposomal Transfection Reagent Yeasen Cat# 40802ES03 Polybrene Beyotime Cat# C0351 Puromycin dihydrochloride Beyotime Cat# ST551 Blasticidin S HCl Beyotime Cat# ST018 DAPI dihydrochloride MedChemExpress Cat# HY-D0814 Critical commercial assays Seamless Cloning Kit Beyotime Cat# D7010 MolPure Gel Extraction Kit Yeasen Cat# 19101ES50 Hieff Canace High-Fidelity DNA Polymerase Yeasen Cat# 10135ES60 MolPure Endo-free Plasmid Mini Kit Yeasen Cat# 19021ES50 23 SuperTaq PCR StarMix (dye) GenStar Cat# A002-10 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 NCI-H1299 ATCC Cat# CRL-5803 Oligonucleotides Seamless Cloning TFEB forward primer: 5′ TCCATAGAAGCCACCATGGCGTCACGCATAGGG 3′ This paper N/A Seamless Cloning TFEB reverse primer: 5′ ATCGAATTCTCTAGACTTGTCGTCATCGTCTTTG 3′ This paper N/A Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCDH-CMV-MCS-GFP-IRES-RFP Modified from Addgene pCDH-Flag-c-Myc Addgene Plasmid Cat# 102626 pCDH-CMV-MCS-GFP-TFEB-IRES-RFP This paper N/A PLKO.1-shNT Addgene Cat# 109012 PLKO.1-shYEATS2 Wang et al. N/A Software and algorithms FlowJo v.10 BD Biosciences https://www.flowjo.com Other 0.45 μm filters BIOFIL Cat# FPE404025 XbaI Beyotime Cat# D6713 EcoRI Beyotime Cat# D6330 BD FACSCanto II BD Biosciences N/A STAR Protocols 6, 104141, December 19, 2025 3 Generate cell lines stably expressing pCDH-CMV-TFEB-GFP-IRES-RFP through lentiviral transduction.

    Modification:

    Article Title: Protocol for monitoring the stability of transcription factor EB using global protein stability assay.
    Article Snippet: Bacterial and virus strains E. coli DH5α cells Thermo Fisher Scientific Cat# 18265017 Chemicals, peptides, and recombinant proteins Penicillin/Streptomycin Thermo Fisher Scientific Cat# 15070063 Fetal bovine serum Sigma-Aldrich Cat# F0193 DMEM Metacell Cat# C1010 RPMI1640 Metacell Cat# C1120 0.25% Trypsin-EDTA solution Metacell Cat# C1201 Liposomal Transfection Reagent Yeasen Cat# 40802ES03 Polybrene Beyotime Cat# C0351 Puromycin dihydrochloride Beyotime Cat# ST551 Blasticidin S HCl Beyotime Cat# ST018 DAPI dihydrochloride MedChemExpress Cat# HY-D0814 Critical commercial assays Seamless Cloning Kit Beyotime Cat# D7010 MolPure Gel Extraction Kit Yeasen Cat# 19101ES50 Hieff Canace High-Fidelity DNA Polymerase Yeasen Cat# 10135ES60 MolPure Endo-free Plasmid Mini Kit Yeasen Cat# 19021ES50 23 SuperTaq PCR StarMix (dye) GenStar Cat# A002-10 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 NCI-H1299 ATCC Cat# CRL-5803 Oligonucleotides Seamless Cloning TFEB forward primer: 5′ TCCATAGAAGCCACCATGGCGTCACGCATAGGG 3′ This paper N/A Seamless Cloning TFEB reverse primer: 5′ ATCGAATTCTCTAGACTTGTCGTCATCGTCTTTG 3′ This paper N/A Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCDH-CMV-MCS-GFP-IRES-RFP Modified from Addgene pCDH-Flag-c-Myc Addgene Plasmid Cat# 102626 pCDH-CMV-MCS-GFP-TFEB-IRES-RFP This paper N/A PLKO.1-shNT Addgene Cat# 109012 PLKO.1-shYEATS2 Wang et al. N/A Software and algorithms FlowJo v.10 BD Biosciences https://www.flowjo.com Other 0.45 μm filters BIOFIL Cat# FPE404025 XbaI Beyotime Cat# D6713 EcoRI Beyotime Cat# D6330 BD FACSCanto II BD Biosciences N/A STAR Protocols 6, 104141, December 19, 2025 3 Generate cell lines stably expressing pCDH-CMV-TFEB-GFP-IRES-RFP through lentiviral transduction.

    Software:

    Article Title: Protocol for monitoring the stability of transcription factor EB using global protein stability assay.
    Article Snippet: Bacterial and virus strains E. coli DH5α cells Thermo Fisher Scientific Cat# 18265017 Chemicals, peptides, and recombinant proteins Penicillin/Streptomycin Thermo Fisher Scientific Cat# 15070063 Fetal bovine serum Sigma-Aldrich Cat# F0193 DMEM Metacell Cat# C1010 RPMI1640 Metacell Cat# C1120 0.25% Trypsin-EDTA solution Metacell Cat# C1201 Liposomal Transfection Reagent Yeasen Cat# 40802ES03 Polybrene Beyotime Cat# C0351 Puromycin dihydrochloride Beyotime Cat# ST551 Blasticidin S HCl Beyotime Cat# ST018 DAPI dihydrochloride MedChemExpress Cat# HY-D0814 Critical commercial assays Seamless Cloning Kit Beyotime Cat# D7010 MolPure Gel Extraction Kit Yeasen Cat# 19101ES50 Hieff Canace High-Fidelity DNA Polymerase Yeasen Cat# 10135ES60 MolPure Endo-free Plasmid Mini Kit Yeasen Cat# 19021ES50 23 SuperTaq PCR StarMix (dye) GenStar Cat# A002-10 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 NCI-H1299 ATCC Cat# CRL-5803 Oligonucleotides Seamless Cloning TFEB forward primer: 5′ TCCATAGAAGCCACCATGGCGTCACGCATAGGG 3′ This paper N/A Seamless Cloning TFEB reverse primer: 5′ ATCGAATTCTCTAGACTTGTCGTCATCGTCTTTG 3′ This paper N/A Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCDH-CMV-MCS-GFP-IRES-RFP Modified from Addgene pCDH-Flag-c-Myc Addgene Plasmid Cat# 102626 pCDH-CMV-MCS-GFP-TFEB-IRES-RFP This paper N/A PLKO.1-shNT Addgene Cat# 109012 PLKO.1-shYEATS2 Wang et al. N/A Software and algorithms FlowJo v.10 BD Biosciences https://www.flowjo.com Other 0.45 μm filters BIOFIL Cat# FPE404025 XbaI Beyotime Cat# D6713 EcoRI Beyotime Cat# D6330 BD FACSCanto II BD Biosciences N/A STAR Protocols 6, 104141, December 19, 2025 3 Generate cell lines stably expressing pCDH-CMV-TFEB-GFP-IRES-RFP through lentiviral transduction.

    Stable Transfection:

    Article Title: Protocol for monitoring the stability of transcription factor EB using global protein stability assay.
    Article Snippet: Bacterial and virus strains E. coli DH5α cells Thermo Fisher Scientific Cat# 18265017 Chemicals, peptides, and recombinant proteins Penicillin/Streptomycin Thermo Fisher Scientific Cat# 15070063 Fetal bovine serum Sigma-Aldrich Cat# F0193 DMEM Metacell Cat# C1010 RPMI1640 Metacell Cat# C1120 0.25% Trypsin-EDTA solution Metacell Cat# C1201 Liposomal Transfection Reagent Yeasen Cat# 40802ES03 Polybrene Beyotime Cat# C0351 Puromycin dihydrochloride Beyotime Cat# ST551 Blasticidin S HCl Beyotime Cat# ST018 DAPI dihydrochloride MedChemExpress Cat# HY-D0814 Critical commercial assays Seamless Cloning Kit Beyotime Cat# D7010 MolPure Gel Extraction Kit Yeasen Cat# 19101ES50 Hieff Canace High-Fidelity DNA Polymerase Yeasen Cat# 10135ES60 MolPure Endo-free Plasmid Mini Kit Yeasen Cat# 19021ES50 23 SuperTaq PCR StarMix (dye) GenStar Cat# A002-10 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 NCI-H1299 ATCC Cat# CRL-5803 Oligonucleotides Seamless Cloning TFEB forward primer: 5′ TCCATAGAAGCCACCATGGCGTCACGCATAGGG 3′ This paper N/A Seamless Cloning TFEB reverse primer: 5′ ATCGAATTCTCTAGACTTGTCGTCATCGTCTTTG 3′ This paper N/A Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCDH-CMV-MCS-GFP-IRES-RFP Modified from Addgene pCDH-Flag-c-Myc Addgene Plasmid Cat# 102626 pCDH-CMV-MCS-GFP-TFEB-IRES-RFP This paper N/A PLKO.1-shNT Addgene Cat# 109012 PLKO.1-shYEATS2 Wang et al. N/A Software and algorithms FlowJo v.10 BD Biosciences https://www.flowjo.com Other 0.45 μm filters BIOFIL Cat# FPE404025 XbaI Beyotime Cat# D6713 EcoRI Beyotime Cat# D6330 BD FACSCanto II BD Biosciences N/A STAR Protocols 6, 104141, December 19, 2025 3 Generate cell lines stably expressing pCDH-CMV-TFEB-GFP-IRES-RFP through lentiviral transduction.

    Expressing:

    Article Title: Protocol for monitoring the stability of transcription factor EB using global protein stability assay.
    Article Snippet: Bacterial and virus strains E. coli DH5α cells Thermo Fisher Scientific Cat# 18265017 Chemicals, peptides, and recombinant proteins Penicillin/Streptomycin Thermo Fisher Scientific Cat# 15070063 Fetal bovine serum Sigma-Aldrich Cat# F0193 DMEM Metacell Cat# C1010 RPMI1640 Metacell Cat# C1120 0.25% Trypsin-EDTA solution Metacell Cat# C1201 Liposomal Transfection Reagent Yeasen Cat# 40802ES03 Polybrene Beyotime Cat# C0351 Puromycin dihydrochloride Beyotime Cat# ST551 Blasticidin S HCl Beyotime Cat# ST018 DAPI dihydrochloride MedChemExpress Cat# HY-D0814 Critical commercial assays Seamless Cloning Kit Beyotime Cat# D7010 MolPure Gel Extraction Kit Yeasen Cat# 19101ES50 Hieff Canace High-Fidelity DNA Polymerase Yeasen Cat# 10135ES60 MolPure Endo-free Plasmid Mini Kit Yeasen Cat# 19021ES50 23 SuperTaq PCR StarMix (dye) GenStar Cat# A002-10 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 NCI-H1299 ATCC Cat# CRL-5803 Oligonucleotides Seamless Cloning TFEB forward primer: 5′ TCCATAGAAGCCACCATGGCGTCACGCATAGGG 3′ This paper N/A Seamless Cloning TFEB reverse primer: 5′ ATCGAATTCTCTAGACTTGTCGTCATCGTCTTTG 3′ This paper N/A Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCDH-CMV-MCS-GFP-IRES-RFP Modified from Addgene pCDH-Flag-c-Myc Addgene Plasmid Cat# 102626 pCDH-CMV-MCS-GFP-TFEB-IRES-RFP This paper N/A PLKO.1-shNT Addgene Cat# 109012 PLKO.1-shYEATS2 Wang et al. N/A Software and algorithms FlowJo v.10 BD Biosciences https://www.flowjo.com Other 0.45 μm filters BIOFIL Cat# FPE404025 XbaI Beyotime Cat# D6713 EcoRI Beyotime Cat# D6330 BD FACSCanto II BD Biosciences N/A STAR Protocols 6, 104141, December 19, 2025 3 Generate cell lines stably expressing pCDH-CMV-TFEB-GFP-IRES-RFP through lentiviral transduction.

    Transduction:

    Article Title: Protocol for monitoring the stability of transcription factor EB using global protein stability assay.
    Article Snippet: Bacterial and virus strains E. coli DH5α cells Thermo Fisher Scientific Cat# 18265017 Chemicals, peptides, and recombinant proteins Penicillin/Streptomycin Thermo Fisher Scientific Cat# 15070063 Fetal bovine serum Sigma-Aldrich Cat# F0193 DMEM Metacell Cat# C1010 RPMI1640 Metacell Cat# C1120 0.25% Trypsin-EDTA solution Metacell Cat# C1201 Liposomal Transfection Reagent Yeasen Cat# 40802ES03 Polybrene Beyotime Cat# C0351 Puromycin dihydrochloride Beyotime Cat# ST551 Blasticidin S HCl Beyotime Cat# ST018 DAPI dihydrochloride MedChemExpress Cat# HY-D0814 Critical commercial assays Seamless Cloning Kit Beyotime Cat# D7010 MolPure Gel Extraction Kit Yeasen Cat# 19101ES50 Hieff Canace High-Fidelity DNA Polymerase Yeasen Cat# 10135ES60 MolPure Endo-free Plasmid Mini Kit Yeasen Cat# 19021ES50 23 SuperTaq PCR StarMix (dye) GenStar Cat# A002-10 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 NCI-H1299 ATCC Cat# CRL-5803 Oligonucleotides Seamless Cloning TFEB forward primer: 5′ TCCATAGAAGCCACCATGGCGTCACGCATAGGG 3′ This paper N/A Seamless Cloning TFEB reverse primer: 5′ ATCGAATTCTCTAGACTTGTCGTCATCGTCTTTG 3′ This paper N/A Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCDH-CMV-MCS-GFP-IRES-RFP Modified from Addgene pCDH-Flag-c-Myc Addgene Plasmid Cat# 102626 pCDH-CMV-MCS-GFP-TFEB-IRES-RFP This paper N/A PLKO.1-shNT Addgene Cat# 109012 PLKO.1-shYEATS2 Wang et al. N/A Software and algorithms FlowJo v.10 BD Biosciences https://www.flowjo.com Other 0.45 μm filters BIOFIL Cat# FPE404025 XbaI Beyotime Cat# D6713 EcoRI Beyotime Cat# D6330 BD FACSCanto II BD Biosciences N/A STAR Protocols 6, 104141, December 19, 2025 3 Generate cell lines stably expressing pCDH-CMV-TFEB-GFP-IRES-RFP through lentiviral transduction.



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    Addgene inc paper n a plko 1 shnt addgene
    Paper N A Plko 1 Shnt Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paper+n+a+plko+1+shnt+addgene/pLKO%2E1-puro-shNT+(Plasmid+%23109012)/pm41076632-47-166-169
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